Difference between revisions of "Part:BBa J01080:Experience"

Latest revision as of 09:29, 18 October 2014

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Applications of BBa_J01080

User Reviews

UNIQ9960aa3a0a9101f1-partinfo-00000000-QINU

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQ9960aa3a0a9101f1-partinfo-00000002-QINU

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Hong_Kong_HKUST_2014

We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction to induce the Pbad promoter that regulates the key to the riboregulator system,, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.

For detailed information and figures, visit HKUST iGEM 2014 team wiki page http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization.

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-We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.
+We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction to induce the Pbad promoter that regulates the key to the riboregulator system,, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.
-For more information, visit HKUST iGEM 2014 team wiki page [[http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization]].
+For detailed information and figures, visit HKUST iGEM 2014 team wiki page [[http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization]].
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