Difference between revisions of "Part:BBa I746909:Experience"

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===Applications of BBa_I746909===
 
===Applications of BBa_I746909===
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====[http://2014.igem.org/Team:Imperial iGEM Team Imperial====
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The iGEM Team Aachen used the biobrick I746909 to test their [http://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chip technology].
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|<html> <img src="https://static.igem.org/mediawiki/parts/d/d3/Aachen_I746909_slower_all.gif" width="480px"></html>
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|'''{{{title|Testing I746909 in sensor chips}}}'''<br />{{{subtitle|I746909 in sensor chips induced with 2µl IPTG and measured with a plate reader. Top chip is not induced, bottom chip is induced with IPTG. }}}
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[[File:IC-2014_glow1.jpg |200px|left|]]
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[[File:IC-2014_glow2.jpg |400px|lright|]]
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We used this part as a template to make our own part (K1321337). We added Freiburg sites onto the existing sfGFP using overhang end primers during PCR. The part we made was sequence confirmed and showed very bright fluorescence, even when fused to our CBDs (cellulose binding domains). 
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====[http://2014.igem.org/Team:Aachen iGEM Team Aachen====
 
====[http://2014.igem.org/Team:Aachen iGEM Team Aachen====

Revision as of 05:59, 2 November 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I746909

[http://2014.igem.org/Team:Imperial iGEM Team Imperial

The iGEM Team Aachen used the biobrick I746909 to test their [http://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chip technology].

Testing I746909 in sensor chips
I746909 in sensor chips induced with 2µl IPTG and measured with a plate reader. Top chip is not induced, bottom chip is induced with IPTG.


IC-2014 glow1.jpg

IC-2014 glow2.jpg

We used this part as a template to make our own part (K1321337). We added Freiburg sites onto the existing sfGFP using overhang end primers during PCR. The part we made was sequence confirmed and showed very bright fluorescence, even when fused to our CBDs (cellulose binding domains).



[http://2014.igem.org/Team:Aachen iGEM Team Aachen

The iGEM Team Aachen used the biobrick I746909 to test their [http://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chip technology].

Testing I746909 in sensor chips
I746909 in sensor chips induced with 2µl IPTG and measured with a plate reader. Top chip is not induced, bottom chip is induced with IPTG.

The biorbick I746909 was also used to test the measurement device [http://2014.igem.org/Team:Aachen/Project/Measurement_Device WatsOn].

Testing I746909 in WatsOn
I746909 in sensor chips induced with 2µl IPTG and measured with WatsOn. Left chip is not induced, right chip is induced with IPTG.

User Reviews

UNIQe4ebed917171b037-partinfo-00000003-QINU UNIQe4ebed917171b037-partinfo-00000004-QINU