Difference between revisions of "Part:BBa K1395002:Design"

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Latest revision as of 03:55, 18 October 2014


sqr gene (sulfide quinone reductase) under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 390


Design Notes

During designing we used RFC10 assembly to create this biobrick by the use of previously existing biobricks. As per the protocol of RFC 10 we were constrained to use pSB1K3 as our backbone to eliminate the chances of false clone as our Part A and Part B were present in pSB1C3. Once cloning was done we digested our clone with EcoR1 and Pst1, and ligated it with pSB1C3 to standardise our part.

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Source

Biobrick Bba_K896000 and Constitutive Promoter (BBA_J23119)


References