Difference between revisions of "Part:BBa K1431824"

Revision as of 19:22, 21 October 2014

amajLime, yellow-green chromoprotein reporter system (Weak Promoter, Weak RBS)

Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.

We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.

Selective Figures

LB agar plate of Biobricks BBa_K1431812 transformed in BL21 after incubating 23h at 37℃

LB agar plate of Biobricks BBa_K1431812 transformed in DH5α after incubating 23h at 37℃

LB broth of Biobricks BBa_K1431812 after incubating 11h at 37℃,180rpm

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Selective Figures===
-<center>https://static.igem.org/mediawiki/parts/6/62/34_916_BL21.jpg</center>
+<center>https://static.igem.org/mediawiki/parts/6/62/31_916_BL21.jpg</center>
 <center>'''LB agar plate of Biobricks BBa_K1431812 transformed in BL21 after incubating 23h at 37℃'''</center>
-<center>https://static.igem.org/mediawiki/parts/3/3f/34_916_dh5.jpg</center>
+<center>https://static.igem.org/mediawiki/parts/3/3f/31_916_dh5.jpg</center>
 <center>'''LB agar plate of Biobricks BBa_K1431812 transformed in DH5α after incubating 23h at 37℃'''</center>