Difference between revisions of "Part:BBa K1431834:Design"

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===Source===
 
===Source===
 +
The following list is the Biobricks we've used in construction and their detailed design information.
  
−
Will be added later
+
{| class="wikitable"
 +
! Part
 +
! Type
 +
! Discription
 +
! Backbone
 +
! Location on the plate
 +
|-
 +
| BBa_J23100
 +
| Promoter
 +
| Strong Promoter
 +
| BBa_J61002 (Amp)
 +
| 17D 2014 Kit Plate 4
 +
|-
 +
| BBa_J23106
 +
| Promoter
 +
| Weak Promoter
 +
| BBa_J61002 (Amp)
 +
| 17P 2014 Kit Plate 4
 +
|-
 +
| BBa_B0031
 +
| RBS
 +
| Weak RBS
 +
| pSB1A2
 +
| 1H 2014 Kit Plate 4
 +
|-
 +
| BBa_B0034
 +
| RBS
 +
| Strong RBS
 +
| pSB1A2
 +
| 1N 2014 Kit Plate 4
 +
|-
 +
| BBa_B0015
 +
| Terminator
 +
| Strong terminator (B0010+B0012)
 +
| pSB1C3
 +
| 3F 2014 Kit Plate 3
 +
|-
 +
| BBa_K592009
 +
| Chromoprotein
 +
| amilCP, blue chromoprotein
 +
| pSB1C3
 +
| 19E 2014 Kit Plate 1
 +
|-
 +
| BBa_K592011
 +
| Chromoprotein
 +
| cjBlue, green chromoprotein
 +
| pSB1C3
 +
| 2I 2014 Kit Plate 4
 +
|-
 +
| BBa_K1033916
 +
| Chromoprotein
 +
| amajLime, yellow-green chromoprote
 +
| pSB1C3
 +
| 6M 2014 Kit Plate 4
 +
|}
  
 
===References===
 
===References===
 +
#[[About_Assembly|Parts Registry Assembly Help]]
 +
#[[Help:Protocols/Transformation|Parts Registry Transformation Guideline]]

Revision as of 03:04, 18 October 2014

amajLime, yellow-green chromoprotein reporter system (Weak Promoter, Strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Biobrick is constructed through 3 steps:

  1. Do double enzyme digestion (XbaI & PstI for BBa_K1033916, SpeI & PstI for BBa_B0034) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
  2. Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for J23106) and do gel extraction, ligation, transformation, picking up single colonies (can select the positive colonies from the colony color), LB broth incubation, plasmid extraction and gel electrophoresis verification.
  3. Do double enzyme digestion (EcoRI-HF & SpeI for Step 2 product, EcoRI-HF & XbaI for B0015) and do gel extraction, ligation, transformation, picking up single colonies (can select the positive colonies from the colony color), LB broth incubation, plasmid extraction and gel electrophoresis verification.
  4. Cryopreserved the rest bacteria broth and send samples for sequencing.

Source

The following list is the Biobricks we've used in construction and their detailed design information.

Part Type Discription Backbone Location on the plate
BBa_J23100 Promoter Strong Promoter BBa_J61002 (Amp) 17D 2014 Kit Plate 4
BBa_J23106 Promoter Weak Promoter BBa_J61002 (Amp) 17P 2014 Kit Plate 4
BBa_B0031 RBS Weak RBS pSB1A2 1H 2014 Kit Plate 4
BBa_B0034 RBS Strong RBS pSB1A2 1N 2014 Kit Plate 4
BBa_B0015 Terminator Strong terminator (B0010+B0012) pSB1C3 3F 2014 Kit Plate 3
BBa_K592009 Chromoprotein amilCP, blue chromoprotein pSB1C3 19E 2014 Kit Plate 1
BBa_K592011 Chromoprotein cjBlue, green chromoprotein pSB1C3 2I 2014 Kit Plate 4
BBa_K1033916 Chromoprotein amajLime, yellow-green chromoprote pSB1C3 6M 2014 Kit Plate 4

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline