Difference between revisions of "Part:BBa K1500010"
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| − | Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants. | + | Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants. |
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Revision as of 16:44, 1 November 2014
Insert non-formatted text here IPTG--GFP--EmrR
Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream with an additional RBS and the EmrR mutator gene. Using a fluctuation analysis mutation rate experiment, XL1Blue bacteria transformed with this construct yielded a mutation rate of 3.14E-09 mutations per cell per culture although mutation rate is dependent on growth conditions and can vary depending on experimental setup. This plasmid appears to incur a growth penalty in transformants.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1424
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870