Difference between revisions of "Part:BBa K1391107:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts. | |
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− | + | ||
===Source=== | ===Source=== |
Latest revision as of 16:15, 30 October 2014
pEXPR_TRE:Syk-TEVp
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 7362
Illegal EcoRI site found at 7403
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 5083
Illegal PstI site found at 5663
Illegal PstI site found at 6452
Illegal PstI site found at 6832 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 7362
Illegal EcoRI site found at 7403
Illegal NheI site found at 3210
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 5083
Illegal PstI site found at 5663
Illegal PstI site found at 6452
Illegal PstI site found at 6832
Illegal NotI site found at 8245 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 7362
Illegal EcoRI site found at 7403
Illegal BglII site found at 6150
Illegal BglII site found at 7483
Illegal BamHI site found at 2854
Illegal BamHI site found at 3328
Illegal BamHI site found at 4632
Illegal BamHI site found at 8440
Illegal XhoI site found at 3538
Illegal XhoI site found at 8225 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 7362
Illegal EcoRI site found at 7403
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 5083
Illegal PstI site found at 5663
Illegal PstI site found at 6452
Illegal PstI site found at 6832 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3580
Illegal EcoRI site found at 4233
Illegal EcoRI site found at 4598
Illegal EcoRI site found at 7362
Illegal EcoRI site found at 7403
Illegal XbaI site found at 4525
Illegal PstI site found at 2677
Illegal PstI site found at 3656
Illegal PstI site found at 3939
Illegal PstI site found at 5083
Illegal PstI site found at 5663
Illegal PstI site found at 6452
Illegal PstI site found at 6832
Illegal NgoMIV site found at 2297
Illegal NgoMIV site found at 3741
Illegal NgoMIV site found at 4024
Illegal AgeI site found at 3456 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7212
Illegal BsaI.rc site found at 1121
Illegal SapI site found at 38
Illegal SapI site found at 3214
Illegal SapI site found at 8672
Design Notes
This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
Human