Difference between revisions of "Part:BBa J63010:Experience"

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Several members of the Silver lab use this plasmid routinely. We find that it is very reliable for standard assemblies. Typically a 5 mL overnight culture of TOP10 cells carrying this plasmid can be mini-prepped to provide ~10 ug of plasmid DNA.   
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Several members of the Silver lab use this plasmid routinely. We find that it is very reliable for standard assemblies. Typically a 5 mL overnight culture of TOP10 cells carrying this plasmid yields ~10 ug of mini-prepped plasmid DNA.   
  
 
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Latest revision as of 15:08, 29 October 2006


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J63010

User Reviews

UNIQe6f031a32edb928b-partinfo-00000000-QINU

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Cajofranklin

Several members of the Silver lab use this plasmid routinely. We find that it is very reliable for standard assemblies. Typically a 5 mL overnight culture of TOP10 cells carrying this plasmid yields ~10 ug of mini-prepped plasmid DNA.

UNIQe6f031a32edb928b-partinfo-00000002-QINU