Difference between revisions of "Part:BBa K1439004"
 
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[[File:OUC-China_K1439004_Main1.jpg]]
 
[[File:OUC-China_K1439004_Main1.jpg]]
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The secondary structure of TAT-H4 fusion protein.
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====The secondary structure of TAT-H4 fusion protein.====

Latest revision as of 02:43, 18 October 2014

TAT-H4

Histone H4 can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
    Illegal XhoI site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 241


OUC-China K1439004 Main1.jpg

The secondary structure of TAT-H4 fusion protein.