Difference between revisions of "Part:BBa K1365006:Design"
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+ | Trmčić, A. et al. (2011) Complete nisin A gene cluster from ''Lactococcus lactis'' M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. ''Genes Genom.'' 33: 217-221 |
Revision as of 02:35, 18 October 2014
NisR and NisK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3'
to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix.
Changed the GTG start codon of NisR into a ATG.
Source
Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.
References
Trmčić, A. et al. (2011) Complete nisin A gene cluster from Lactococcus lactis M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. Genes Genom. 33: 217-221