Difference between revisions of "Part:BBa K1509003"
(→Usage and Biology) |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1509003 short</partinfo> | <partinfo>BBa_K1509003 short</partinfo> | ||
| − | The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they | + | The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measured the activities of the CP promoter in <em>E. coli</em>.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:34, 18 October 2014
CP25 is a highly active,constitutive promoter
The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measured the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.
Usage and Biology
fig1.CDS7 gene was under control of CP25 promoter.
Result
fig2.The E.coli in the feild of TEM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]