Difference between revisions of "Part:BBa K1520520"
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PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter | PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter | ||
− | It is part of our test of suicide circuit by gold ions | + | It is part of our test of suicide circuit by gold ions. |
[[File:suicidewithoutgold.jpg]] | [[File:suicidewithoutgold.jpg]] |
Revision as of 02:10, 18 October 2014
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.
this is part of our part BBa_K1520509 [1] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.
From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]