Difference between revisions of "Part:BBa K1491015"

Latest revision as of 03:51, 18 October 2014

Codon Optimized KillerRed

This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 357

@@ Line 6: / Line 6: @@
 The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)
-https://static.igem.org/mediawiki/2014/thumb/b/b0/Colonies.jpg/800px-Colonies.jpg
+<center>https://static.igem.org/mediawiki/2014/thumb/0/0a/LacSuperoxides.jpg/389px-LacSuperoxides.jpg</center>
 <hr>
-https://static.igem.org/mediawiki/2014/thumb/e/e0/RFU.jpg/736px-RFU.jpg
+<center>https://static.igem.org/mediawiki/2014/thumb/d/dc/ConstitutiveSuperoxides.jpg/386px-ConstitutiveSuperoxides.jpg</center>