Difference between revisions of "Part:BBa K1548001"

Latest revision as of 01:25, 18 October 2014

Hsp60 promoter (M. bovis) and RBS (mycobacteriophage TM4)

Strong constitutive promoter from M. bovis fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.

References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. Sequence and Features Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 202
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1548001 short</partinfo>
-Strong constitutive promoter from ''M. bovis'' and fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.
+Strong constitutive promoter from ''M. bovis'' fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.
 References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4.