Difference between revisions of "Part:BBa K1520019"
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Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter | Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter | ||
It produces luxI when there is enough IPTG. | It produces luxI when there is enough IPTG. | ||
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[[File:k1520019.jpg]] | [[File:k1520019.jpg]] | ||
Latest revision as of 01:21, 18 October 2014
Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
From left to right, Marker(1Kb), Pcons2-rbs-lacI-Ter, Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
Illegal BglII site found at 2222 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]