Difference between revisions of "Part:BBa K1444006"
Sarahtownes (Talk | contribs) |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1444006 short</partinfo> | <partinfo>BBa_K1444006 short</partinfo> | ||
| − | A composite part consisting of the constitutive promoter Pveg <partinfo>BBa_K823003</partinfo>, followed by three copies of the repressible promoter C1P22 <partinfo>BBa_R0053</partinfo>, and ending with the consensus RBS from ''B. subtilis'' <partinfo>BBa_K090505</partinfo>. | + | A composite part consisting of the constitutive promoter Pveg<partinfo>BBa_K823003</partinfo>, followed by three copies of the repressible promoter C1P22 <partinfo>BBa_R0053</partinfo>, and ending with the consensus RBS from ''B. subtilis'' <partinfo>BBa_K090505</partinfo>. |
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.The triplicate repressible promoter allows for stronger repression. | This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.The triplicate repressible promoter allows for stronger repression. | ||
Revision as of 01:06, 18 October 2014
Composite promoter and consensus B. subtilis RBS - C2P22 x3
A composite part consisting of the constitutive promoter PvegBBa_K823003, followed by three copies of the repressible promoter C1P22 BBa_R0053, and ending with the consensus RBS from B. subtilis BBa_K090505.
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.The triplicate repressible promoter allows for stronger repression.
IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pUC57 vector.
Usage and Biology
This part was cloned in front of RFP and characterized as BBa_K1444022. See that page for more details.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]