Difference between revisions of "Part:BBa K1502001:Design"

Revision as of 23:53, 17 October 2014

accA portion of Acyl-CoA Ligase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 579
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 517
Illegal SapI.rc site found at 714

Design Notes

The PCR process included X and P overhangs in order to include into the pSB1C3 plasmid.

Source

This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene.

References

(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.

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	===References===		===References===
		+
		+	(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.