Difference between revisions of "Part:pSB1AK8:Design"
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===Design Notes=== | ===Design Notes=== | ||
Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). | Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). | ||
| + | This vector was very important for us, because of its terminator. We cloned all our composite parts in this vector, so all composite parts end with terminator. | ||
===Source=== | ===Source=== | ||
Revision as of 13:55, 29 October 2006
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). This vector was very important for us, because of its terminator. We cloned all our composite parts in this vector, so all composite parts end with terminator.
Source
The source for eukaryotic terminator is pcDNA3 by Invitrogen.