Difference between revisions of "Part:BBa K1328000"
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Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose. | Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose. | ||
− | [[File:Tsinghua_Project_Microbe_Fig_1.png|600px|thumb|alt=| | + | [[File:Tsinghua_Project_Microbe_Fig_1.png|600px|thumb|alt=Alt|Figure 1.EGFP fluorescence observed in yeast cells cultured in a) %2 glucose, and b) 3% glycerol. c) Flow cytometry analysis: Cell count vs. EGFP intensity. Red: 2% glucose; Cyan: 3% glycerol.]] |
− | + | [[File:Tsinghua_Project_Microbe_Fig_2.png|600px|thumb|alt=Alt|Figure 2. Mean EGFP fluorescence measured by flow cytometry (FACS) in yeast cells, a) induced by different levels of glucose, and b) different incubation durations.]] | |
− | [[File:Tsinghua_Project_Microbe_Fig_2.png|thumb|alt=Alt| | + | |
− | Figure 2. Mean EGFP fluorescence measured by flow cytometry (FACS) in yeast cells, a) induced by different levels of glucose, and b) different incubation durations. | + | |
Revision as of 22:52, 17 October 2014
Part:BBa_K1328000 (HXT1-CMVmini) is a glucose-sensitive promoter of yeast origin and shown to function in both yeast and human cells. Exclusively activated by high glucose concentrations.
Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter.
HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator.
Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.
Figure 3. Flow cytometry analysis of glucose-induced EGFP expression in HEK293 cells cultured in various glucose concentrations and citrate (as non-glucose carbon source). Plotted is frequency vs. EGFP intensity.
For full characterization of this part, visit Team Tsinghua 2014's project wiki: http://2014.igem.org/Team:Tsinghua/Project/Microbe