Difference between revisions of "Part:BBa K1328000"
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Figure 2. Mean EGFP fluorescence measured by flow cytometry (FACS) in yeast cells, a) induced by different levels of glucose, and b) different incubation durations. | Figure 2. Mean EGFP fluorescence measured by flow cytometry (FACS) in yeast cells, a) induced by different levels of glucose, and b) different incubation durations. | ||
Revision as of 22:47, 17 October 2014
Part:BBa_K1328000 (HXT1-CMVmini) is a glucose-sensitive promoter of yeast origin and shown to function in both yeast and human cells. Exclusively activated by high glucose concentrations.
Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter.
HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator.
Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.
Figure 1. EGFP fluorescence observed in yeast cells cultured in a) %2 glucose, and b) 3% glycerol. c) Flow cytometry analysis: Cell count vs. EGFP intensity. Red: 2% glucose; Cyan: 3% glycerol.
Figure 2. Mean EGFP fluorescence measured by flow cytometry (FACS) in yeast cells, a) induced by different levels of glucose, and b) different incubation durations.
Figure 3. Flow cytometry analysis of glucose-induced EGFP expression in HEK293 cells cultured in various glucose concentrations and citrate (as non-glucose carbon source). Plotted is frequency vs. EGFP intensity.
For full characterization of this part, visit Team Tsinghua 2014's project wiki: http://2014.igem.org/Team:Tsinghua/Project/Microbe