Difference between revisions of "Part:BBa K1328000"
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Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.
 
Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.
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[[File:Tsinghua_Project_Microbe_Fig_1.png]]
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For full characterization of this part, visit Team Tsinghua 2014's project wiki:
 
For full characterization of this part, visit Team Tsinghua 2014's project wiki:
 
http://2014.igem.org/Team:Tsinghua/Project/Microbe
 
http://2014.igem.org/Team:Tsinghua/Project/Microbe

Revision as of 22:39, 17 October 2014

Part:BBa_K1328000 (HXT1-CMVmini) is a glucose-sensitive promoter of yeast origin and shown to function in both yeast and human cells. Exclusively activated by high glucose concentrations. 2014-HXT1%2BCMV-forget.png

Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter.

HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator.

Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.


Tsinghua Project Microbe Fig 1.png



For full characterization of this part, visit Team Tsinghua 2014's project wiki: http://2014.igem.org/Team:Tsinghua/Project/Microbe