Difference between revisions of "Part:BBa K592100:Experience"

Latest revision as of 22:13, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K592100

iGEM11_Uppsala-Sweden. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU.

iGEM14_Carnegie_Mellon. We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm).

User Reviews

UNIQe714166c10653245-partinfo-00000000-QINU UNIQe714166c10653245-partinfo-00000001-QINU

@@ Line 14: / Line 14: @@
 <p>
 '''iGEM14_Carnegie_Mellon'''.
-We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell line (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at ex/em = 399nm/456nm.
+We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm).
 </p>
 https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png