Difference between revisions of "Part:BBa K1413024"

 
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<partinfo>BBa_K1413024 short</partinfo>
 
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bphR1 promoter, from Pseudomonas pseudoalcaligenes KF707, activates the bph genes of biphenyls degradation, in presence of bphR2 protein and biphenyl.
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In our study, the aim was to detect the presence of polychlorinated biphenyls (PCBs) with RFP fluorescence.
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This part is composed by bphR1 promoter, from Pseudomonas pseudoalcaligens KF707, which allows the transcription of the genes of biphenyl degradation. In our project, genes of degradation was replaced by RFP gene to detect the compound.
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  <a href="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" width="202px;" class="thumbimage"/>
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    <a href="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" class="internal" title="Enlarge">
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
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    <center><u><b>Figure 1: Schema of BBa_K1413024 construction.</u></b></center>
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<br/><u>Mechanism explanation:</u> In absence of PCBs, bphR2 (BBa_K1413021) is bound to bphR1 promoter which activate the transcription of RFP but in very low expression.  
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In presence of PCBs, when compound diffuses into the media, it binds to bphr2 protein which undergoes a conformational change that permits to activate more stronger bphR1 promoter and increase the transcription of RFP.
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  <a href="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" class="image">
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" width="402px;" class="thumbimage"/>
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    <a href="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" class="internal" title="Enlarge">
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
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    <center><u><b>Figure 2 : Mechanism of PCB biosensor with the system bphR2/bphR1 gene</u></b></center>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:47, 1 November 2014

bphR1-RBS-RFP-Terminator
This part is composed by bphR1 promoter, from Pseudomonas pseudoalcaligens KF707, which allows the transcription of the genes of biphenyl degradation. In our project, genes of degradation was replaced by RFP gene to detect the compound.

IMAGE
Figure 1: Schema of BBa_K1413024 construction.

Mechanism explanation: In absence of PCBs, bphR2 (BBa_K1413021) is bound to bphR1 promoter which activate the transcription of RFP but in very low expression. In presence of PCBs, when compound diffuses into the media, it binds to bphr2 protein which undergoes a conformational change that permits to activate more stronger bphR1 promoter and increase the transcription of RFP.

IMAGE
Figure 2 : Mechanism of PCB biosensor with the system bphR2/bphR1 gene

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
    Illegal AgeI site found at 995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139