Difference between revisions of "Part:BBa K1413024"
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<partinfo>BBa_K1413024 short</partinfo> | <partinfo>BBa_K1413024 short</partinfo> | ||
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− | bphR1 promoter, from Pseudomonas | + | <br/> |
− | In our | + | This part is composed by bphR1 promoter, from Pseudomonas pseudoalcaligens KF707, which allows the transcription of the genes of biphenyl degradation. In our project, genes of degradation was replaced by RFP gene to detect the compound. |
+ | <br/> | ||
+ | <div class="center"> | ||
+ | <div class="thumb tnone"> | ||
+ | <div class="thumbinner" style="width:502px;"> | ||
+ | <a href="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" class="image"> | ||
+ | <img alt="IMAGE" src="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" width="202px;" class="thumbimage"/> | ||
+ | </a> | ||
+ | <div class="thumbcaption"> | ||
+ | <div class="magnify"> | ||
+ | <a href="https://static.igem.org/mediawiki/parts/f/f4/Evry2014_Plasmid_bphr1.jpg" class="internal" title="Enlarge"> | ||
+ | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
+ | </a> | ||
+ | </div> | ||
+ | <center><u><b>Figure 1: Schema of BBa_K1413024 construction.</u></b></center> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br/><u>Mechanism explanation:</u> In absence of PCBs, bphR2 (BBa_K1413021) is bound to bphR1 promoter which activate the transcription of RFP but in very low expression. | ||
+ | In presence of PCBs, when compound diffuses into the media, it binds to bphr2 protein which undergoes a conformational change that permits to activate more stronger bphR1 promoter and increase the transcription of RFP. | ||
+ | <br/><br/> | ||
+ | <div class="center"> | ||
+ | <div class="thumb tnone"> | ||
+ | <div class="thumbinner" style="width:502px;"> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" class="image"> | ||
+ | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" width="402px;" class="thumbimage"/> | ||
+ | </a> | ||
+ | <div class="thumbcaption"> | ||
+ | <div class="magnify"> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/3/3f/Igemevry_2014_Sch%C3%A9ma_g%C3%A9n%C3%A9ral_pcb.jpg" class="internal" title="Enlarge"> | ||
+ | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
+ | </a> | ||
+ | </div> | ||
+ | <center><u><b>Figure 2 : Mechanism of PCB biosensor with the system bphR2/bphR1 gene</u></b></center> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br/> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 21:47, 1 November 2014
bphR1-RBS-RFP-Terminator
This part is composed by bphR1 promoter, from Pseudomonas pseudoalcaligens KF707, which allows the transcription of the genes of biphenyl degradation. In our project, genes of degradation was replaced by RFP gene to detect the compound.
Mechanism explanation: In absence of PCBs, bphR2 (BBa_K1413021) is bound to bphR1 promoter which activate the transcription of RFP but in very low expression. In presence of PCBs, when compound diffuses into the media, it binds to bphr2 protein which undergoes a conformational change that permits to activate more stronger bphR1 promoter and increase the transcription of RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 156
Illegal BamHI site found at 239
Illegal XhoI site found at 46 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
Illegal AgeI site found at 995 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 139