Difference between revisions of "Part:BBa K1372000"
| Line 2: | Line 2: | ||
<partinfo>BBa_K1372000 short</partinfo> | <partinfo>BBa_K1372000 short</partinfo> | ||
| − | This BB is the result of the assembly of two BB: | + | This BB is the result of the assembly of two BB: |
| + | |||
- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). | - BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). | ||
- BBa_K228001(lien): for the transcription of SupD-tRNA. | - BBa_K228001(lien): for the transcription of SupD-tRNA. | ||
| + | |||
[[File:Paris Saclay BB2000.png|center]] | [[File:Paris Saclay BB2000.png|center]] | ||
Revision as of 19:09, 17 October 2014
salicylate inducible suppressing system
This BB is the result of the assembly of two BB:
- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). - BBa_K228001(lien): for the transcription of SupD-tRNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1312