Difference between revisions of "Part:BBa K1462260"

Latest revision as of 19:36, 17 October 2014

Gal1+cox6+Erg10+cyc1

This part express the first enzyme ----thiolase. Erg10 is expressed under control of GAL1 promoter and cyc1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1378
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1462260 short</partinfo>
-This part is constructed as a control group to BBa_K1462110 to identify Erg10 has been directed to the mitochondria. The GFP is fused at the C-terminus of Erg10 to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
+This part express the first enzyme ----thiolase. Erg10 is expressed under control of GAL1 promoter and cyc1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.
+[[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
 <!-- Add more about the biology of this part here