Difference between revisions of "Part:BBa K1351034"

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<partinfo>BBa_K1351034 short</partinfo>
 
<partinfo>BBa_K1351034 short</partinfo>
  
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"This part was generated in a modified version of RFC10, and has the following prefix and suffix:
 
  
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{|
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This part was generated in RFC10, and has the following prefix and suffix:  
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|prefix with EcoRI, NotI, XbaI and NgoMIV:
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|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>
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              Prefix                                                                  Suffix
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|-
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5' - GAATTC GCGGCCGC T TCTAGA G aagaagagata T ACTAGT A GCGGCCG CTGCAG - 3'
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|suffix with AgeI, SpeI, NotI and PstI:
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      EcoRI   NotI     XbaI                                         SpeI     NotI  PstI
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|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
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|}
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Sites of restriction enzymes generating compatible overhangs have the same color:
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<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:03, 17 October 2014

LMU Bacillus RBS collection 7


This part was generated in RFC10, and has the following prefix and suffix: 

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aagaagagata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]