Difference between revisions of "Part:BBa K1328002"

Revision as of 15:57, 17 October 2014

HXT1-CMVmini-EGFP-tADH1

HXT-1+EGFP+ADH1 consists of HXT-1+CMV promoter, EGFP and terminator ADH1.

HXT-1+CMV promoter controls EGFP expression under the regulation of glucose.

EGFP is used here for testing of HXT1+CMV promoter and will be replaced by insulin gene.

HXT1-CMVmini-EGFP-tADH1

This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.

S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.

Sequence and Features