Difference between revisions of "Part:BBa K896008:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 20: | Line 20: | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| − | lac promoter ([https://parts.igem.org/Part:BBa_R0010 BBa_R0010]) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts [ | + | lac promoter ([https://parts.igem.org/Part:BBa_R0010 BBa_R0010]) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K896008 BBa_K1342005]. |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K896008 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K896008 EndReviews</partinfo> | ||
Revision as of 14:24, 17 October 2014
- Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.
- This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.
Applications of BBa_K896008
It can be used to detect cadmium ion when ligating with GFP.
User Reviews
UNIQcf6140d8b9cce359-partinfo-00000000-QINU
|
No review score entered. iGEM Nagahama_2014 |
lac promoter (BBa_R0010) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts BBa_K1342005. |
UNIQcf6140d8b9cce359-partinfo-00000002-QINU