Difference between revisions of "Part:BBa K1509002:Design"
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===Design Notes=== | ===Design Notes=== | ||
It was attained from ''Bacillus sp''. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: | It was attained from ''Bacillus sp''. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below: | ||
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P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ | P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’ | ||
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P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ | P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ | ||
And the PCR runs under the settings as follows: | And the PCR runs under the settings as follows: | ||
| − | + | [[File:Flocculation_PCR.png]] | |
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Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25. | Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25. | ||
Revision as of 01:20, 18 October 2014
Design Notes
It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:
P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’
P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’ And the PCR runs under the settings as follows:
Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
Sourse
It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.