Difference between revisions of "Part:BBa K1331001"
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This is an improvement of [https://parts.igem.org/Part:BBa_K317998 Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen.  
 
This is an improvement of [https://parts.igem.org/Part:BBa_K317998 Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen.  
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A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
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A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.  
  
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It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.  
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RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part [https://parts.igem.org/Part:BBa_K1331004 rhlB] encodes subunit B.
  
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The coding sequence of our part is similar with [https://parts.igem.org/Part:BBa_K317998 Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
 
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===Usage and Biology===
 
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RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part is [https://parts.igem.org/Part:BBa_K1331004 rhlB].
 
 
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Revision as of 15:08, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This is an improvement of Part:BBa_K317998 designed by iGEM10_Tokyo-NoKoGen. A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.This part encodes rhamnosyltransferase I subunit A, and the other part rhlB encodes subunit B.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478