Difference between revisions of "Part:BBa K1415201"

Revision as of 13:43, 20 October 2014

Pcons+B0034+PBAN(Bombyx mori)+B0034+BFP+J61048

Fig.5-2 Shows the entering times of moths(Spodoptera litura) in different conditions. Entering times is defined as the times that moths get into the device. In this experiment, the moth might get out of our device due to it was just a model without trap, such as nets for keeping the moths. However, we can still clearly see the magic power of our pyramidal device by using blue light with PBAN treatment, which attracted more moths to get into the device than the other experiment (only blue light treatment). In addition, we can even observe a unique periodicity of attracting moths only in blue light with PBAN treatment. We consider this phenomenon is related to the physiology of the female moths' rut situation.

Fig.5-2 The entering number (into our pyramidal device) per hour shows that the combination of blue light, PBAN, and our device is indeed magically powerful in insect attraction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 909
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1415201 short</partinfo>
-To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.
+'''Fig.5-2''' Shows the entering times of moths(''Spodoptera litura'') in different conditions. Entering times is defined as the times that moths get into the device. In this experiment, the moth might get out of our device due to it was just a model without trap, such as nets for keeping the moths. However, '''we can still clearly see the magic power of our pyramidal device by using blue light with PBAN treatment, which attracted more moths to get into the device than the other experiment (only blue light treatment).''' In addition, we can even observe a unique periodicity of attracting moths only in blue light with PBAN treatment. We consider this phenomenon is related to the physiology of the female moths' rut situation.
+[[File:Magic Power of Our Device.png|thumb|750px|center|'''Fig.5-2''' The entering number (into our pyramidal device) per hour shows that the combination of blue light, PBAN, and our device is indeed magically powerful in insect attraction.]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
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 <partinfo>BBa_K1415201 parameters</partinfo>
 <!-- -->
-[[File:Pcons(J23101) + RBS(B0034) + PBAN(BM) + RBS(B0034) + BFP(K592100) + Ter(J61048) .png|thumb|left|1200px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(BM) + BFP + Term. ]]
-[[File:BLBM.png|thumb|left|1200px|'''Fig.1-2''' The growth curve of E.coli containing Pcons + RBS +  PBAN(BM) + RBS + BFP + Ter plasmid (control is the competent cells which can not emit blue light). ]]
-[[File:MDBM.png|thumb|left|1200px|'''Fig.1-3'''Modeling result of Pcons + RBS + PBAN(BM) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(BM) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias. ]]