Difference between revisions of "Part:BBa K1331003"
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The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence. | The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:37, 17 October 2014
RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.
Usage and Biology
Acyl-homoserine-lactone synthase.Required for the synthesis of autoinducer molecules C4-HSL.Together with rhlR positively regulates the promoter of rhlAB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 89
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 221