Difference between revisions of "Part:BBa K660004:Experience"
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<I>Exeter University 2014</I> | <I>Exeter University 2014</I> | ||
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Revision as of 12:35, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K660004
User Reviews
UNIQc40693b874c4ed49-partinfo-00000000-QINU
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Exeter University 2014 |
As part of the Exeter 2014 project iLOV was used as a reporter in two constructs. iLOV was used to test the effectiveness of a NemR upstream intergenic region (https://parts.igem.org/Part:BBa_K1398005) in part BBa_1398004 (https://parts.igem.org/Part:BBa_K1398004). Part BBa_1398007 (https://parts.igem.org/Part:BBa_K1398007) used iLOV to test the effectiveness of a NemR recognizing promoter, part BBa_1398008 (https://parts.igem.org/Part:BBa_1398008). (Full article available at:http://2014.igem.org/Team:Exeter/Detection#TheNemRpromoterconstruct%28BBa_K1398007%29) As part of their project the fluorescence topography of iLOV was mapped. The response of iLOV to a low power light source was also explored. The fluorescence response of iLOV was greatest when excited with photons of wavelength 449±1nm whilst photon emissions where detected at a wavelength of 494±2nm.
(Full article available at: http://2014.igem.org/Team:Exeter/iLOVCharacterisation) |
UNIQc40693b874c4ed49-partinfo-00000002-QINU