Difference between revisions of "Part:BBa K1462140"

Latest revision as of 13:04, 17 October 2014

Gal1+Ccr+GFP+ADH1

This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Ccr to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Ccr is expressed in the cytoplasm

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1203
Illegal BsaI.rc site found at 2549

Revision as of 12:23, 17 October 2014 (view source) Weapon (Talk \| contribs) ← Older edit		Latest revision as of 13:04, 17 October 2014 (view source) Weapon (Talk \| contribs)
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−	[[File:GCG.jpg\|200px\|left\|frame\|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that ~~Erg10~~ is expressed in the cytoplasm]]	+	[[File:GCG.jpg\|200px\|left\|frame\|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Ccr is expressed in the cytoplasm]]