Difference between revisions of "Part:BBa K1331001"

Revision as of 13:03, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This is an improvement of Part:BBa_K317998 designed by iGEM10_Tokyo-NoKoGen. A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.

The coding sequence of our part is similar with Part:BBa_K317998 designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.This part encodes rhamnosyltransferase I subunit A, and the other part is rhlB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1331001 short</partinfo>
-This part encodes rhamnosyltransferase I subunit A. Another subunit is [https://parts.igem.org/Part:BBa_K1331004 rhlB].
+This is an improvement of [https://parts.igem.org/Part:BBa_K317998 Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen.
+A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
 It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
@@ Line 9: / Line 10: @@
 ===Usage and Biology===
-RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
+RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part is [https://parts.igem.org/Part:BBa_K1331004 rhlB].
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