Difference between revisions of "Part:BBa K1351030"

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|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
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|prefix with EcoRI, NotI, XbaI and NgoMIV:
 
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>
 
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>
 
|-
 
|-

Revision as of 15:57, 17 October 2014

LMU Bacillus RBS collection 3

"This part was generated in a modified version of RFC10, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI and NgoMIV: GAATTCGCGGCCGCTTCTAGATGGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]