Difference between revisions of "Part:BBa K1331004"

Revision as of 11:46, 17 October 2014

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit B.

It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.

The coding sequence of our part is similar with Part:BBa_K317999 [1] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

RhlB together with rhlA [2] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 758
Illegal NgoMIV site found at 871
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 387

@@ Line 3: / Line 3: @@
 This part encodes rhamnosyltransferase I subunit B.
 It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.
 The coding sequence of our part is similar with [[Part:BBa_K317999]][https://parts.igem.org/Part:BBa_K317999] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.