Difference between revisions of "Part:BBa K1351030"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1351028 short</partinfo>
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<partinfo>BBa_K1351030 short</partinfo>
  
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This part was generated in a modified version of RFC10, and has the following prefix and suffix:  
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"This part was generated in a modified version of RFC10, and has the following prefix and suffix:  
  
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{||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color:
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{|
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|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
 +
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>
 +
|-
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|suffix with AgeI, SpeI, NotI and PstI:
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|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
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|}
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Sites of restriction enzymes generating compatible overhangs have the same color:
  
 
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.
 
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.

Revision as of 11:49, 17 October 2014

LMU Bacillus RBS collection 3

"This part was generated in a modified version of RFC10, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI, SD and NgoMIV: GAATTCGCGGCCGCTTCTAGATGGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]