Difference between revisions of "Part:BBa K1351029"
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| − | <partinfo> | + | <partinfo>BBa_K1351028 short</partinfo> |
| − | + | This part was generated in a modified version of RFC10, and has the following prefix and suffix: | |
| − | {| | + | {||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color: |
| − | |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: | + | |
| − | |<span style= | + | |
| − | |- | + | |
| − | |suffix with AgeI, SpeI, NotI and PstI: | + | |
| − | |<span style= | + | |
| − | |} | + | |
| − | Sites of restriction enzymes generating compatible overhangs have the same color: | + | |
| − | <span style= | + | <span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined. |
Revision as of 11:27, 17 October 2014
LMU Bacillus RBS collection 1
This part was generated in a modified version of RFC10, and has the following prefix and suffix: