Difference between revisions of "Part:BBa K1415105"

Line 22: Line 22:
 
[[File:HALFSL.png|thumb|left|600px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(SL) ]]
 
[[File:HALFSL.png|thumb|left|600px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(SL) ]]
  
[[File:KDASL2.png|thumb|left|600px|'''Fig.1-2'''  Protein Electrophoresis of Pcons + RBS +  PBAN(SL) (control: plasmid of Pcons+RBS) Each peptide of PBAN is an around 30 amino acids, so we can see the band of PBANs at 2~4 kDa.]]
+
[[File:KDASL3.png|thumb|left|600px|'''Fig.1-2'''  Protein Electrophoresis of Pcons + RBS +  PBAN(SL) (control: plasmid of Pcons+RBS) Each peptide of PBAN is an around 30 amino acids, so we can see the band of PBANs at 2~4 kDa.]]

Revision as of 11:17, 17 October 2014

Pcons+B0034+PBAN(Spodoptera litura)

This part has a constitutive promoter, so our PBAN(Spodoptera litura) can constitutive express.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]



Fig.1-1 Biobrick of Pcons + RBS + PBAN(SL)
Fig.1-2 Protein Electrophoresis of Pcons + RBS + PBAN(SL) (control: plasmid of Pcons+RBS) Each peptide of PBAN is an around 30 amino acids, so we can see the band of PBANs at 2~4 kDa.