Difference between revisions of "Part:BBa K1509001"

Revision as of 11:16, 17 October 2014

A bi-directional promoter affected by SmtB protein

smt O-P is a promoter which has two inverted repeats, designated S1/S2 and S3/S4, in the overlapping promoter/operator sites between gene smtA and gene smtB. The full promoter/operator DNA binds two SmtB dimmers forming a S1/S2-SmtB:SmtB-S3/S4 bridge complex. After binding with smtB, smt O-P represses the expression of downstream genes.

Usage and Biology

In our project, we wanted to use pigments as reporters in our designed genetic construction which can be recognizable by the naked eyes. According to the previous work, we have chosen an identified pigment in the Registry: the biobrick of amilCP (BBa_K592009) was used as reporter gene in our metal detection device. After exchanging the biobrick part with SmtA in smtB-OP-smtA device, the pigment gene was under control of metal-induced promoter (smtB-OP).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-In our project, we wanted to use pigments as reporters in our designed genetic construction which can be recognizable by the naked eyes. According to the previous work, we have chosen an identified pigment in the Registry: the biobrick of amilCP ([[BBa_K592009]][https://parts.igem.org/Part:BBa_K592009]) was used as reporter gene in our metal detection device. After exchanging the biobrick part with SmtA in smtB-OP-smtA device, the pigment gene was under control of metal-induced promoter (smtB-OP).
+In our project, we wanted to use pigments as reporters in our designed genetic construction which can be recognizable by the naked eyes. According to the previous work, we have chosen an identified pigment in the Registry: the biobrick of amilCP (BBa_K592009) was used as reporter gene in our metal detection device. After exchanging the biobrick part with SmtA in smtB-OP-smtA device, the pigment gene was under control of metal-induced promoter (smtB-OP).
 [[File:Fig3.smtB-OP-amilCP.png‎]]