Difference between revisions of "Part:BBa K1331001"

Revision as of 11:11, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit A. RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA. The coding sequence of our part is similar with Part:BBa_K317998 [1] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1331001 short</partinfo>
-This part encodes rhamnosyltransferase I subunit A.RhlA together with rhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in ''Pseudomonas aeruginosa''.
+This part encodes rhamnosyltransferase I subunit A. RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.
-It includes the whole coding sequence and a part of downstream noncoding gene of RhlA.
+It includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
-The coding sequence is similar with [[Part:BBa_K317998]][https://parts.igem.org/Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
+The coding sequence of our part is similar with [[Part:BBa_K317998]][https://parts.igem.org/Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen, but a mutation has been done to remove the PstI restriction site(at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.
 <!-- Add more about the biology of this part here