Difference between revisions of "Part:BBa K1431101:Design"
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| − | + | The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR. | |
| − | The | + | |
===References=== | ===References=== | ||
Revision as of 08:21, 17 October 2014
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design the primers and PCR from plasmid.
Primer Forward: T TCTAGA TGGGATCAAGACTGGACAAGA
Primer Reverse: AAAACTGCAG CGGCCGC T ACTAGT A CCGAAGCCCAACCTTTCATA
Source
The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.