Difference between revisions of "Part:BBa K1334002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | A gfp reporter gene was added downstream so that we can know whether the P-formaldehyde works or not in the presence of formaldehyde by detecting the relative fluorescence intensity. | ||
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+ | [[File:P-Formaldehyde+GFP.png]] | ||
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+ | Figure 3 A GFP reporter is added downstream of the formaldehyde promoter. | ||
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+ | We incubated E.coli strain DH5a with the above plasmid (P-formaldehyde plus GFP)and stimulated the experimental group with 1mM formaldehyde when the OD600 value reached approximately one, which means E.coli comes into mid-log phase. And then we collected bacteria at certain time to measure the fluorescence and the absorbance. | ||
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+ | From the Figure 4, we can see significant increase in relative fluorescence intensity after formaldehyde stimulation, which shows that our coloration system works well. The Growth Condition curve in Figure 5 shows that the difference of the fluorescence/absorbance is not generated by the absorbance difference. | ||
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+ | [[File:Figure 4 The function test of the formaldehyde promoter which shows our part BBa_K1334002 works well.png]] | ||
+ | [[File:Figure 5 The growth condition of E.coli strain DH5a after formaldehyde stimulation.png]] | ||
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Revision as of 04:03, 17 October 2014
HxlR+P-formaldehyde
A right promoter which can sense formaldehyde.There is a left promoter which can erpress the HxlR protein.The HxlR protein can enhance the function of the P-formaldehyde.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]