Difference between revisions of "Part:BBa K1323010"
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This construct contains the reporter protein coding sequence for the yellow fluorescent protein (YFP). It has been codon optimized for Staphylococcus aureus by Bose et al. (2013). YFP has an excitation peak at 514 nm and an emission peak at 527 nm (needs ref). We order the synthesis of this coding sequence from Biobasic. This is a reliable reporter in S.aureus. (Malone et al., 2009) | This construct contains the reporter protein coding sequence for the yellow fluorescent protein (YFP). It has been codon optimized for Staphylococcus aureus by Bose et al. (2013). YFP has an excitation peak at 514 nm and an emission peak at 527 nm (needs ref). We order the synthesis of this coding sequence from Biobasic. This is a reliable reporter in S.aureus. (Malone et al., 2009) | ||
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Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224. | Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:48, 17 October 2014
YFP Coding Sequence
This construct contains the reporter protein coding sequence for the yellow fluorescent protein (YFP). It has been codon optimized for Staphylococcus aureus by Bose et al. (2013). YFP has an excitation peak at 514 nm and an emission peak at 527 nm (needs ref). We order the synthesis of this coding sequence from Biobasic. This is a reliable reporter in S.aureus. (Malone et al., 2009)
References
Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]