Difference between revisions of "Part:BBa K1480005"

Revision as of 00:48, 17 October 2014

TetR generator (Promoter J23111 )

This is a TetR protein generator regulated by the constitutive promoter J23111.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

K1140003 [[1]] K1480003 [[2]] k145201 [[3]] K1480004 [[4]] K1480005 [[5]]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 [[File:TetR repressors(FCB UANL 2014).JPG|thumb|center|400px|It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.]]
+K1140003 [[https://parts.igem.org/Part:BBa_K1140003]]
+K1480003 [[https://parts.igem.org/Part:BBa_K1480003]]
+k145201  [[https://parts.igem.org/Part:BBa_K145201]]
+K1480004 [[https://parts.igem.org/Part:BBa_K1480004]]
+K1480005 [[https://parts.igem.org/Part:BBa_K1480005]]
 <!-- Add more about the biology of this part here