Difference between revisions of "Part:BBa K1520506"
| Line 18: | Line 18: | ||
e) A visible photograph of d. | e) A visible photograph of d. | ||
| + | |||
| + | [[File:k1520506.jpg]] | ||
| + | |||
| + | From left to right, Marker(DL10000), OMPA-golB. | ||
| + | The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:57, 18 October 2014
PgolTS-golS-PgolB
PgolTS-golS-PgolB works as a promoter, which will result in the expression of downstream gene in the presence of gold ions. PgolTS works like a constant promoter, producing gols which is inhibitor of PgolB. Once combining gold ions, gols will stop inhibitor PgolB. So, when there are gold ions, the CDS after this part will be expressed.
a) Genetic organization of the gol locus in the S. typhimurium LT2 genome.
b) Genetic organization of the gold inductive RFP expression plasmid in E. coli.
c) E. coli cells containing the gold-induced RFP expression plasmid with a concentration of 20 mM each of metal ions, or without metal ions induction.
d) Fluorescence measurement of E. coli cells containing the gold-induced RFP expression plasmid after gradient concentrations of HAuCl4 (Au3+) induction.
e) A visible photograph of d.
From left to right, Marker(DL10000), OMPA-golB. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]