Difference between revisions of "Part:BBa K1463520"

Revision as of 21:57, 16 October 2014

E1010 RFP in reverse

Part E1010, RFP, in reverse.

Usage and Biology

This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site BBa_K1463560 with transcription driven by the reverse promoter BBa_K1463501 in BBa_K1463000.

See BBa_K1463560 and BBa_K1463000 for more details.

Fig. 1: Red fluorescence of colonies containing BBa_K1463560 or BBa_I742130 reversed RBSs "downstream" of the reversed rfp gene BBa_K1463520 transcribed from the PhiC31 integrase switch BBa_K1463000

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 34
Illegal AgeI site found at 146
1000
COMPATIBLE WITH RFC[1000]

Revision as of 21:55, 16 October 2014 (view source) Scolloms (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 21:57, 16 October 2014 (view source) Scolloms (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details.		See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details.

−	[[Image:reverse-rbs-rfp.jpg\|thumb\|500px\|center\|'''Fig. 1:''' Red fluorescence of colonies containing [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [https://parts.igem.org/Part:BBa_I742130 BBa_I742130] reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 ~~K1463000~~]]]	+	[[Image:reverse-rbs-rfp.jpg\|thumb\|500px\|center\|'''Fig. 1:''' Red fluorescence of colonies containing [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [https://parts.igem.org/Part:BBa_I742130 BBa_I742130] reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000]]]