Difference between revisions of "Part:BBa K1541017"
| Line 6: | Line 6: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
====Quorum Sensing==== | ====Quorum Sensing==== | ||
| − | RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'' which produces the | + | RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'' which produces the molecule [https://parts.igem.org/File:ETH_Zurich_2014_C4-HSL.png ''N''-butyryl-L-HSL] (C4-HSL). |
====RBS Engineering==== | ====RBS Engineering==== | ||
Revision as of 21:12, 16 October 2014
rhlI with optimized RBS
This part contains the part C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR, (C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.
Usage and Biology
Quorum Sensing
RhlI is the autoinducer synthetase from Pseudomonas aeruginosa which produces the molecule N-butyryl-L-HSL (C4-HSL).
RBS Engineering
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]