Difference between revisions of "Part:BBa K1489000:Experience"

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<partinfo>BBa_K1489000 AddReview number</partinfo>
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<partinfo>BBa_K1489000 1</partinfo>
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<I>Username</I>
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<I>Iowis Zhu</I>
 
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Enter the review inofrmation here.
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Expression tests
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BBa_K148900 was transformed into chemically competent DH5-alpha cells and plated overnight with chloramphenicol challenge. One colony was incubated in 5 mL LB media + 34 ug/mL chloramphenicol and grown to mid-log phase at 37oC. Arabinose was added to a final concentration of 0.2% to initiate protein production and cells were re-incubated at 37 oC for 21 hours. Cells were then pelleted and lysed with 10% SDS solution. Cell lysate was centrifuged at 13200 rpm in a tabletop centrifuge for 1 hour to separate soluble and insoluble fractions. Both fractions were loaded on an SDS-PAGE gel for western blot analysis using anti-His antibodies.
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[[File:BovineGalectinExpression.jpg]]
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Clear band of correct size can be seen in the soluble induced fraction, while no band is seen in the uninduced cells.
 
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Revision as of 20:20, 16 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1489000

User Reviews

UNIQe4ef46e8e02a00ea-partinfo-00000000-QINU UNIQe4ef46e8e02a00ea-partinfo-00000001-QINU